Coding
Part:BBa_K2984039
Designed by: Dimitri Schumacher Group: iGEM19_Humboldt_Berlin (2019-10-16)
Revision as of 10:27, 17 October 2019 by Chlamy Dima (Talk | contribs)
L1c-PsaD-PETase-3xHA-RBCS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linkerr, the PETase enzyme, a HA tag, and the Rbcs2 terminator. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1705
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1244
Illegal PstI site found at 1705 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 968 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1705
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1705
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
chassis | C. reinhardtii |
protein | PETase |
tag | 3xHA |