Coding

Part:BBa_K2984035

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 12:30, 16 October 2019 by Registry (Talk | contribs)

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L1c-Psad-PETase-YFP-His-RbcS2

L1c plasmid containing PETase Enzyme fused to mVEnus fluorescent marker for fluorecence meausrements of PETase concentration and His-tag for purification. The parts are flanked by the Psad promoter and the Rbcs2 terminator. The construct is desgined to measure the erxpression level of PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1244
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
    Illegal NgoMIV site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisC. reinhardtii
emission528nm
excitation515 nm
proteinPETase