Coding

Part:BBa_K2984033

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 09:46, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-AR-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containg arylsulfatase secretion signal ARS and mVenus fluorescent marker which are flanked by AR promoter and Rbcs2 terminator. The construct is designed to test for the efficency of the secretion Signal and the expression rates of the AR Promoter in C.reinhardtii with fluorescence intensity measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal PstI site found at 953
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisC.reinhardtii
emission528nm
excitation517 nm