Coding
Part:BBa_K2984033
Designed by: Darius Rauch Group: iGEM19_Humboldt_Berlin (2019-10-16)
L1c-AR-ARS-YFP-Rbcs2
L1c plasmid containg arylsulfatase secretion signal and mVEnus fluorescent marker which are flanked by AR Promoter and Rbcs2 Terminator. The construct is designed to test for the efficency of the secretion Signal and the expression rates of the AR Promoter in C.reinhardtii with fluorescence intensity measurements.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 953
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 268
Illegal PstI site found at 953 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 953
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 953
Illegal NgoMIV site found at 1430 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
chassis | C.reinhardtii |
emission | 528nm |
excitation | 517 nm |