Coding

Part:BBa_K2984030

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 09:18, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-Psad-ARS-YFP-SP20-Rbcs2

This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1 construc containg Arylsulfatase secretion Signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> fluorescent marker, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20</a> glycomodule. These parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None