DNA

Part:BBa_K2961006

Designed by: Muhammad Nahin Khan   Group: iGEM19_CMUQ   (2019-10-16)
Revision as of 03:11, 17 October 2019 by Registry (Talk | contribs)

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Template DNA sequence coding for HBB rs334 mutation (A to T) in humans

This sequence encodes for the HBB gene with the rs334 mutation, which is the most common mutation that leads to the sickle cell anemia phenotype (Glu6Val). One of the nucleotides of the gene was modified so that the Cpf1 PAM site could be added adjacent to a target site for our gRNA, but the overall template largely remains the same.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 174


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Categories
Parameters
None