Part:BBa_K2961000

CFTR Mutant Gene Segment (rs75389940_G)

This part encodes for a guide RNA that can be used with Cpf1. The sequence includes a crRNA repeat, known as the fixed loop, to allow Lab Cas12a to recognize the gRNA (different manufacturers may produce Cas12a that are optimized for recognizing different fixed loops; the sequence posted here is optimized for NEB Cas12a). The sequence also includes a segment that is specific to the CFTR gene, specifically the SNP variant rs75389940_G, which is a common allele in the Middle East responsible for the prevalence of cystic fibrosis.

This part may be used to simply manufacture this gRNA. It can be used in conjunction with template DNA extracted from patients or synthesized in a lab to allow Cas12a to recognize and bind to the mutated CFTR gene. This may then be paired with further downstream effects (such as Cas12a activity causing fluorescence of some kind) to detect DNA samples that have the genetic disease.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]