DNA

Part:BBa_K2961001:Design

Designed by: Muhammad Nahin Khan   Group: iGEM19_CMUQ   (2019-10-13)
Revision as of 03:08, 14 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


CFTR Wildtype gene segment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One must ensure that the target sequence does not contain the Cas12a PAM sequence, but rather that in the human genome, the PAM sequence of Cas12a is just upstream of it. (The PAM sequence for Cas12a is TTTN)


Source

he fixed loop part was adapted from the NEB website, as per their instructions (https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a). The segment that is specific to the CFTR gene wild type variant was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares.

References