Measurement

Part:BBa_K3286046:Design

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
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mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1723


Design Notes

The native promoter regions have been used for this construct, including the 5'UTR's. The start of either the mRFP and the GRP are at same position of


Source

Bacteriophage Lambda sequences were obtained from NCBI Reference Sequence: NC_001416.1 and synthetically synthesised in a gBlock. The mRFP and the GFP were obtained from iGEM supervisor.

References