Composite

Part:BBa_K3105666

Designed by: Jinwen Yuan   Group: iGEM19_Uppsala_Universitet   (2019-08-26)
Revision as of 11:08, 26 August 2019 by Registry (Talk | contribs)

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AsPink with ribosome standby site upstream of RBS

AsPink (BBa_k1033906) with a ribosome standby site upstream of the RBS (J23110). We added the ribosome standby site by inverse PCR mutagenesis and achieved higher expression than the original part in Escherichia coli when expressed in a low copy plasmid (pSB3K3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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