Coding

Part:BBa_K3286045

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 14:19, 16 October 2019 by Registry (Talk | contribs)

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Monomeric red fluorescent protein (mRFP)

Red fluorescent protein (RFP) and Green fluorescent protein (GFP) downstream of bacteriophage Lambda promoters, UTR's and operator regions OL and OR.

This construct contains the regulatory regions and promoters of bacteriophage Lambda and has been used to asses a for the potential inhibition of bacteriophage LAmbda by CRISPRi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None