Coding

Part:BBa_K2936007

Designed by: Weng Jiaqi   Group: iGEM19_ZJUT-China   (2019-10-09)
Revision as of 16:05, 16 October 2019 by MQii (Talk | contribs) (Source)


LacZ alpha

An alpha peptide chain encoded by a gene LacZ

Description

Short description: Reporter gene.
The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ).

Source

E. coli

Design consideration

LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.

Mechanism

T--ZJUT-China--igem-part01fanying.png

Experiment

Usage and Biology

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Categories
Parameters
None