Coding

Part:BBa_K3040100

Designed by: Lai, Si-Ying   Group: iGEM19_NTHU_Taiwan   (2019-10-15)
Revision as of 15:23, 16 October 2019 by TALENTTIEN (Talk | contribs)


Lipase-Pseudomonas sp 7323.

Lipase from Antarctic cold-tolerant Pseudomonas sp. 7323 can catalyze the hydrolysis of triacylglycerols to glycerol and monoacylglycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 259
    Illegal NgoMIV site found at 1294
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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