Primer

Part:BBa_K2982007

Designed by: Tsang Hoi Yeung, Ocean   Group: iGEM19_HK_GTC   (2019-10-15)
Revision as of 15:24, 15 October 2019 by Registry (Talk | contribs)

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Forward primer with BBa_J61124 for amplifying BBa_E0040

A primer that contains RBS"BBa_J61124" which is 60 base pairs long that can be used to amplify the GFP part BBa_E0040 by PCR. It can be used to characterize the RBS activity easily by measuring OD.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None