Composite

Part:BBa_K3278001

Designed by: Ailin Zhou   Group: iGEM19_ShanghaiTech_China   (2019-10-13)
Revision as of 15:28, 13 October 2019 by Registry (Talk | contribs)

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pDawn with T7 promoter

Change one of the promoters of pDawn system lacIq to T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
    Illegal NgoMIV site found at 241
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 628
    Illegal NgoMIV site found at 1122
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1230
    Illegal AgeI site found at 460
    Illegal AgeI site found at 1588
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1683
    Illegal BsaI.rc site found at 571


[edit]
Categories
Parameters
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