Composite
Part:BBa_K3239009:Design
Designed by: Xingyu Zhou Group: iGEM19_ShanghaiFLS_China (2019-10-15)
pMIT1-PRM1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2034
Illegal SpeI site found at 793
Illegal PstI site found at 2556 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2034
Illegal NheI site found at 3815
Illegal SpeI site found at 793
Illegal PstI site found at 2556 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2034
Illegal BglII site found at 1387
Illegal BamHI site found at 2917
Illegal BamHI site found at 3836 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2034
Illegal SpeI site found at 793
Illegal PstI site found at 2556 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2034
Illegal SpeI site found at 793
Illegal PstI site found at 2556 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
the parts were fused together via Gibson assembly, hence there's no scar.
Source
P. pastoris GS115