Part:BBa_K2927012
DNA template of crRNA 3 for LbCas12a
crRNA is crucial for the CRISPR Cas system. It is the short sequence that combines with Cas12a protein and forms a functional complex. Once crRNA binds with Cas12a protein, the complex capable to target and cleave targeted double-stranded DNA sequence (cis cleavage activity) and the cleavage of double-stranded DNA activates the cleavage of non-specific ssDNA (trans cleavage activity). In our project, we use crRNA for detecting a part sequence of vp72 membrane protein of African swine fever virus (ASFV).
When crRNA 3 binds with Cas12a protein, the protein may carry out cis-trans cleavage activity once it recognizes its target. By the way, though crRNA 3 is derived from ASFV, there is no possibility for virulence. The sequence of crRNA 3 is derived from p72 which is the capsid of ASFV. Therefore, the sequence of crRNA 3 is too short to be a functional protein. Thus, there is probably no risk for the spread out of ASFV from our project. Therefore, the design of this gene expression was reported in the check-in form and got approved.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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