Coding
Tse2

Part:BBa_K314200

Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger   Group: iGEM10_Washington   (2010-10-18)
Revision as of 03:12, 23 October 2010 by Siegeljb (Talk | contribs)

Toxin Tse2

A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the Pseudomonas aeruginosa type 6 secretion system. Tse2 has an immunity protein, Tsi2, that, when expressed in conjunction with Tse2 prevents cell death. [http://2010.igem.org/Team:Washington/Gram_Negative Find more information here on our wiki!.]

Reference showing that Tse2 is toxic, Tsi2 is the antitoxin, and that these proteins are transferred into target Gram-negative organisms via a secretion system: "A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria" [http://www.ncbi.nlm.nih.gov/pubmed/20114026]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli/nissle
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None