Coding

Part:BBa_K3187028

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 10:21, 15 October 2019 by Hannahrainer (Talk | contribs)


Sortase A7M (Ca2+-independent variant)

Sortase A7M (SrtA7M) is a calcium-independent transpeptidase derived from the enzyme Sortase A from Staphylococcus aureus.1 Similar to Sortase A, SrtA7M connects two peptides covalently, one with a poly-glycine (poly-G) at the N-terminus and one with LPXTG motif at the C-terminus. It acts by cleaving between Gly and Thr of the LPXTG motif and catalysing the formation of an amide bond to the poly-G sequence.2 For the expression and purification of SrtA7M, a part containing T7 promoter, a lac operator and a ribosomal binding site upstream and a 6xHis-Tag downstream of the coding sequence were added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part/winner
Parameters
None