Part:BBa_K3102044:Design

Electron transfer improvement (ACS, ZWF, GND, PGL)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 2933
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 2933
  Illegal NotI site found at 2814
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3490
  Illegal XhoI site found at 408
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 2933
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 2933
  Illegal NgoMIV site found at 2206
  Illegal NgoMIV site found at 2923
  Illegal NgoMIV site found at 5493
  Illegal AgeI site found at 837
  Illegal AgeI site found at 4245
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 3975
  

Design Notes

none

Source

RibA : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331

RibB: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG10465

RibD: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11321

RibE: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11322

RibC: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406

RibF: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11079

AWS: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11448

ZWF: Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

GND: Corynebacterium glutamicum, sequence found through GenBank https://biocyc.org/gene?orgid=CORYNE&id=G18NG-11035-MONOMER#tab=TU

GLD: E.coli, sequence found through GenBank https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?from=798586&to=799581&report=fasta

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.