Composite

Part:BBa_K3102032:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 09:52, 12 October 2019 by SDUQUESNE (Talk | contribs) (→‎Design Notes)


T7-RBS-ZWF-Ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 810
    Illegal NotI site found at 691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 810
    Illegal NgoMIV site found at 83
    Illegal NgoMIV site found at 800
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated a Thymine into Cysteine at the 844bp position (T844C) because of the PstI enzyme (e.g. BBa_K3102008)

Source

Corynebacterium glutamicum, sequence found through Ebi https://www.ebi.ac.uk/ena/data/view/BAB98969

Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

"Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate." Zhao J., Baba T., Mori H., Shimizu K. Metab. Eng. 6:164-174(2004)