Part:BBa_K3205005
luxPR_4G12T
This promoter expresses downstream gene weakly. The expression of this promoter could be up-regulated by the activation of LuxR activator protein which complexed with autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein and two molecules of the signal compound homoserine lactone (HSL) can form a complex. This complex could bind to a palindromic site of the promoter and increase the rate of the transcription. We can design a plasmid which contain a LuxR sequence on the upstream and a gene of interest on the downstream of the promoter. When we want to express the protein of interest, we can add homoserine lactone (HSL) in the medium. The promoter was improved from BBa_R0062. We mutate the T of the fourth base to G, and the C of the twelfth to T. Comparing to the original part, the new part needs higher homoserine lactone (HSL) concentration to activate this promoter，thus decreasing its leakage.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
| None |