Part:BBa_K2980009

CIB1-GCN(4)-mEGFP-FUSLCD

The fusion protein can induce consistant phase-separation in cell once well expressed. Under blue light stimulation, cry2-mcherry (Part:BBa K2980006) will be recruited into its phase.

Light stimulation

To achieve the response to light stimulation, light-sensitive proteins, CIB1(Part:BBa K2980002) and cry2(Part:BBa K2980000), are fused to our phase separation elements and application elements, respectively. When exposed to 488nm laser, CIB1 and cry2 will bind to each other. Since CIB1 is primarily amplified in the compartment formed by phase separation elements (BBa K2980009), cry2 would be recruited to phase, as the switch turns on. Therefore, the distribution of enzyme or other proteins fused to cry2 would be altered by light stimulation.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 112
  
• 1000
  COMPATIBLE WITH RFC[1000]