RNA

Part:BBa_K2965031

Designed by: Wenxiao Li   Group: iGEM19_ZJU-China   (2019-10-15)
Revision as of 16:54, 15 October 2019 by LIWENXIAO (Talk | contribs)

Lba-crRNA-HPV16-1

This part is a crRNA of LbaCas12a which is complementary to HPV16 L1 sequence. There is a a 5' TTTN PAM on the DNA strand opposite the target sequence. When LbaCas12a is guided by this crRNA, once it recognize its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
Parameters
None