Coding
YFP B5-B5

Part:BBa_K2984020:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-15)
Revision as of 10:57, 15 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


YFP - Yellow fluorescent protein for detection B5-B5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codonoptimization of part, Use of promoter, terminator respectively that originates from targeted locus.


Source

Promoter ist part of 5'UTR of photosystemI reaction center subunit II-1. The terminator originates form 3'UTR Ribulose-1,5-bisphosphat-carboxylase.

References