Composite

Part:BBa_K2963032:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-19)
Revision as of 15:13, 13 October 2019 by GHX (Talk | contribs) (→‎References)


racE- encoding racemase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The strength of racE gene expression affects the proportion of D/L glutamate monomer in γ-PGA. In our project, we wanted to regulate the expression of the racE gene to produce γ-PGA with different D/L-glutamate monomer ratios. Tac Promoter contains a kind of operator gene called lacO. We changed the number of operator gene in tac promoter assembling other basic parts to form our composite parts: BBa_K2963032 and BBa_K2963033. The experiment process please visit our EXPERIMENT page.

Source

racE from Bacillus Genome.

References

1. Keitarou K. Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis[J]. Microbiology, 2004, 9(150): 2911-2920.

2. Feng Jiang, Gaofu Qi, Zhixia Ji. Expression of glr gene encoding glutamate racemase in Bacillus licheniformis WX-02 and its regulatory effects on synthesis of poly-γ-glutamic acid[J]. Biotechnology Letters, 2011, 33: 1837-1840.