Coding
SAHS

Part:BBa_K2623007

Designed by: Yingming Zhu   Group: iGEM18_XMU-China   (2018-09-28)


SAHS coding region

Summary

Secreted heat soluble protein acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. The secretory TDP protein of water bear is used to improve the resistance of the bacteria under the conditions such as dehydration.

A separate fragment is 510bp
CAHS Fig1.png

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We transformed the plasmid to the E.coli(BL21), which is always used to express proteins with high efficience to verify the SAHS protein was produced successfully with a small scale.Besides, whether the SAHS protein with a signal peptide could be secreted was determined by SDS-PAGE.

Fig.1 The marker is on the lest, followed by our control group ( the BL21 with the empty plasmid), and the third well is the concentrated supernatant. The unit of the marker is "Kd".


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 218
    Illegal XhoI site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 497
    Illegal SapI.rc site found at 492


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Categories
Parameters
None