Composite
Part:BBa_K3096042
Designed by: Patrick Mueller, Famke Baeurle, Lina Widerspick, Jakob Keck Group: iGEM19_Tuebingen (2019-10-15)
Exendin-4 with a N-terminal secretion tag and a C-terminal cell penetrating peptide
Exendin-4 is a GLP-1 analogon out of Heloderma suspectum and functions as an Incretin mimetic. It is fused with a N-termianl secretion tag to allow it to be secreted by a bacterium. Additionally, a C-terminal cell penetrating peptide was added to ensure that Exendin-4 can pass through the intestinal cells and enter the bloodstream in order to reach the pancreas where it enhances the Insulin secretion. The CPP in this composite part is Penetratin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 250
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |