Composite

Part:BBa_K3096044

Designed by: Patrick Mueller, Famke Baeuerle, Lina Widerspick, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-15)
Revision as of 09:16, 15 October 2019 by Registry (Talk | contribs)

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Glucose dependent expression system of Exendin-4 with a N-terminal secretion tag and a CPP

This is the whole Expression System of Exendin-4. The system responds to glucose via the carbon catabolite repression system which initiates the transcription of the TetR gene when glucose levels are low. TetR binds to the TetR promoter inhibiting the Expression of Downstream genes, in this case our modified Exendin-4.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None