Plasmid

Part:BBa_K3041014

Designed by: Jo-Anne Verschoor   Group: iGEM19_Leiden   (2019-10-07)
Revision as of 12:49, 7 October 2019 by Registry (Talk | contribs)

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ipET28

The pET28 plasmid adjusted to the RFC100 biobrick system of iGEM, thereby, allowing for overproduction of proteins. The plasmid contains a thrombin site and a His-tag allowing for His-tag purification of the produced protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
    Illegal BamHI site found at 58
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal NgoMIV site found at 1788
    Illegal NgoMIV site found at 1948
    Illegal NgoMIV site found at 4995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2868


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Categories
Parameters
None