Coding

Part:BBa_K3187026

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 12:02, 15 October 2019 by Franziska (Talk | contribs)


mCherry Fluorescence Protein

name coat protein with LPETGG in pET24
base pairs 705
molecular weight 26.6 kDa
origin synthetic, derived from Discosoma sp.
properties Red fluorescent, Ex λ: 587nm, Em λ: 610 nm

mCherry is a red fluorescent protein, which is derived from Discosoma sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.[1]

Figure 1: Crystal Structure of mCherry.


References

  1. Nathan Shaner, Robert Campbell, Paul Steinbach, Ben Giepmans, Amy Palmer and Roger Tsien, Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein, Nature Biotechnology, 2004, 22: 1567-1572 [1]
  2. Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None