Regulatory

Part:BBa_K2890001

Designed by: Jin-Shu Yang   Group: iGEM18_HFLS_ZhejiangUnited   (2018-10-07)
Revision as of 10:07, 15 October 2019 by SUNYUHAN (Talk | contribs) (We added the reporter gene GFP to the downstream of the promoter, then detected the sensitivity and tolerance of the promoter to formaldehyde using BL21 as chassis.)


optimized frmR

A optimized frmR promoter for formaldehyde detection.


File:K2890001 contributions.docx


Contributions from Yuhan Sun, JNFLS 2019.

We added the reporter gene GFP to the downstream of the promoter, then detected the sensitivity and tolerance of the promoter to formaldehyde using BL21 as chassis. We cultured E.coli BL21 transformed with the plasmid （PfrmR-GFP）and added different concentration formaldehyde to different groups when the OD600 = 1, then we collected bacteria at certain time to measure the fluorescence and the absorbance. From the Fig. 1, we found that 0.8 mM formaldehyde is the best concentration for expression reporter protein GFP.

File:K2890001 contributions.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 551


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