DNA

Part:BBa_K3176020:Design

Designed by: Tara Fournier   Group: iGEM19_Pasteur_Paris   (2019-10-15)
Revision as of 09:33, 15 October 2019 by Tarafrnr (Talk | contribs) (→‎References)


Specific aptamer for E. faecium


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to deal with the sequencing of our aptamer candidates and select the one with the highest affinity and specificity for E. faecium.


Source

BBa_K3176020 was made from a DNA library synthesized by Eurofins Genomics and selected using the Whole-cell SELEX method.

References

Campbell, R. E., et al. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. doi:10.1073/pnas.082243699

Falord, M., et al. (2011). Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways. PloS one, 6(7), e21323. doi:10.1371/journal.pone.0021323

Aucher, W. et al. (2004. Differences in mesentericin secretion systems from two Leuconostoc strains. FEMS Microbiology Letters, 232(1), pp. 15–22. doi: 10.1016/S0378-1097(04)00011-4.