Coding

Part:BBa_K3140003

Designed by: Fahad Ali   Group: iGEM19_Sydney_Australia   (2019-10-12)
Revision as of 06:56, 14 October 2019 by Fahad3li (Talk | contribs)


PsiM - Norbaeocystin methyltransferase from Psilocybe cubensis

PsiM is a norbaeocystin methyltransferase, which catalyses the conversion of norbaeocystin to psilocybin.

Usage and Biology

PsiM is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in Escherichia coli <add strain> in a <expression system>.

Fig. 1: N,N-dimethylation of norbaeocystin to psilocybin, mediated by PsiM. Intermediates not shown. Two S-adenosyl-L-methionine moieties are consumed, releasing two S-sdenosyl-L-homocysteine moieties as a by-product. Source: KEGG


ADD: -SDS-PAGE - pCW - LC/MAS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None