Coding
Part:BBa_K3190101:Design
Designed by: Ojas Tulsyan Group: iGEM19_UCopenhagen (2019-10-12)
G protein-coupled estrogen receptor (GPER/GPR30) CDS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 750
Design Notes
The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae.
Source
Homo sapiens