Part:BBa_K143032

EpsE Molecular Clutch Gene of B. subtilis

The epsE gene of the exopolysaccharide synthesis operon of B. subtilis has been suggested to function in a manor similar to a molecular clutch#1. If expressed inside a cell it will prevent flagellar movement causing the cell to no longer be able to swim effectively and instead only tumble. As such EpsE could potentially be used as a controller of B. subtilis movement.

Though the EPS operon is normally repressed in B. subtilis, if EpsE is synthetically expressed it would be beneficial for the original copy of epsE to be knocked out. This can be achieved by integrating over the EesE gene with the epsE integration Biobricks (BBa_K143005 and BBa_K143006) which contain 2 in-frame stop codons.

Although many bacterial flaggelar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than B. subtilis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 762
  Illegal AgeI site found at 512
  
• 1000
  COMPATIBLE WITH RFC[1000]