Part:BBa_K3187018

PolyG Tag (GGGG) for Sortase-mediated Ligation

• Name: PolyG tag
• bp: 12
• Da: 300
• Properties: second sequence for Sortase A

Introduction:

The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif and a N-terminal poly glycine motif ^[1][2][3]. The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction.

[[Image:https://2019.igem.org/wiki/images/5/52/T--TU_DARMSTADT--invitro_UZ_TEM.png|450px|thumb|left|'''Figure 1:''' Fermentation]]

Sequence:

GGCggaggcggt

References:

[1] Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering ChemBioChem

[2] Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation

[3] Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering

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Usage and Biology

<img src=”https://2019.igem.org/wiki/images/5/52/T--TU_DARMSTADT--invitro_UZ_TEM.png” alt="Italian Trulli">

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]