RBS
RBS-GsiB
Part:BBa_K143020
Designed by: James Chappell Group: iGEM08_Imperial_College (2008-09-15)
GsiB ribosome binding site (RBS) for B. subtilis
GsiB is an endogenous ribosome binding site from B.subtilis. The sequence of the gsiB ribosome binding site is AAAGGAGG which is complementary to the sequence UUUCCUCC from the 3' region of the 16s rRNA from B.subtilis. GsiB is an endogenous ribosome binding site (RBS) from B.subtilis. The sequence of the gsiB ribosome binding site is AAAGGAGG which is complementary to the sequence UUUCCUCC from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//rbs/prokaryote/constitutive/miscellaneous
//ribosome/prokaryote/bsubtilis
//chassis/prokaryote/bsubtilis
//direction/forward
//regulation/constitutive
//ribosome/prokaryote/bsubtilis
//chassis/prokaryote/bsubtilis
//direction/forward
//regulation/constitutive
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