Composite

Part:BBa_K3102029

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 08:32, 12 October 2019 by SDUQUESNE (Talk | contribs)


T7-RBS-Riboflavin-Ter

This T7-Riboflavin-ter composite composes of RibA (BBa_K3102000) RibB (BBa_K3102001) RibD (BBa_K3102002) RibE (BBa_K3102003) RibC (BBa_K3102004) is used to overexpress the flavin production. This constructs is enriched with primer, T7 promoters, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1013
    Illegal BglII site found at 2110
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 354
    Illegal PstI site found at 3318
    Illegal AgeI site found at 201
    Illegal AgeI site found at 903
    Illegal AgeI site found at 945
    Illegal AgeI site found at 1488
    Illegal AgeI site found at 1863
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None