Composite

Part:BBa_K3110002:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 05:24, 14 October 2019 by Registry (Talk | contribs)

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lldP+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2805
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2242


Design Notes

lldD has an EcoRI site, which we couldn't eliminate and hence the part needs further improvement.


Source

This has been amplified from the genome.

References