Composite

Part:BBa_K3111502:Design

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-30)
Revision as of 11:16, 14 October 2019 by Matas deveikis (Talk | contribs) (→‎References)


TmEncH_DARPin929_StrepII + miniSOG_TP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 171
    Illegal BglII site found at 586
    Illegal BamHI site found at 950
    Illegal BamHI site found at 2101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1677
    Illegal BsaI.rc site found at 2321
    Illegal SapI.rc site found at 520
    Illegal SapI.rc site found at 551


Design Notes

-


Source

-

References

[1] Xu S, Chisholm AD. Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG. Sci Rep [Internet]. 2016 Feb [cited 2019 Oct 14];6(1). Available from: http://www.nature.com/articles/srep21271

[2] Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, et al. Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry. 2016 Jun 21;55(24):3461–

[3] Camacho-Leal MP, Sciortino M, Cabodi S. ErbB2 Receptor in Breast Cancer: Implications in Cancer Cell Migration, Invasion and Resistance to Targeted Therapy, Breast Cancer - From Biology to Medicine. Phuc Van Pham, IntechOpen.