Coding
bFMO

Part:BBa_K3018000

Designed by: Kang Yunzhe   Group: iGEM19_Tongji_China   (2019-10-14)
Revision as of 07:24, 14 October 2019 by Handles (Talk | contribs)


Bacterial flavin-containing monooxydase, codon-optimized

This part encodes a bacterial flavin-containing monooxygenase(FMO), which can catalyze the oxidation of indole. We had the codon-optimized for E. coli. You can use this part to produce indigo through indoxyl and leucoindigo in E. coli. Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates should be blue when it works.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None