Part:BBa_K3140000

PsiD - Tryptophan decarboxylase from Psilocybe cubensis

PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.

• NCBI
• UniProt: P0DPA6
• EC number: 4.1.1.105

Usage and Biology

PsiD is a native enzyme obtained from the Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in Escherichia coli <add strain> in a <expression system>.

Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO₂ is released as a byproduct. Source: KEGG

Fig. 2: Decarboxylation of 4-hydroxy-L-tryptophan to 4-hydroxytryptamine, mediated by PsiD. CO₂ is released as a byproduct. Source: KEGG

ADD:

- SDS-PAGE IMAGE - LC/MS - pUS250 and pET-28c(+)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]