Coding

Part:BBa_K3112002

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-09-12)
Revision as of 12:10, 12 September 2019 by Registry (Talk | contribs)

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dCas9

The first part of Cas9.We made a point mutation and codon optimization in real synthesis and transformed it into dcas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1156


[edit]
Categories
Parameters
None