Composite

Part:BBa_K2963033

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-20)
Revision as of 05:18, 20 September 2019 by Registry (Talk | contribs)

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Ptac(two lacOs)-racE- Producing γ-PGA with different D/L monomer ratio

This part contains the racE gene for glutamate racemase from Bacillus. This enzyme mainly catelyse the reation of L-Glumate to D-Glumate.In our project, we express this enzyme by using the "Ptac-lacO-lacO-rbs-racE-T7t" part in order to reduce the leaked expression of racE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 971
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None