Coding

Part:BBa_K2973020

Designed by: Leandros Tsiotos, Vasiliki Kavvatha   Group: iGEM19_Thessaly   (2019-09-28)
Revision as of 20:42, 28 September 2019 by Registry (Talk | contribs)

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TEM-optimized beta-lactamase / J23100

Beta-lactamase is an enzyme found widely in the procaryots, and confers resistance to ampicillin. This composite part comprises of the TEM-optimized beta-lactamase CDS (BBa_I757010), regulated by the 119 constitutive Anderson Family Promoter (BBa_J23100) and a universal RBS (BBa_B0034). Transcription is terminated by double terminator (BBa_B0015). This allows for enhanced protein expression in E. coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 783


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Categories
Parameters
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