RNA

Part:BBa_K2923008

Designed by: claire husser   Group: iGEM19_Strasbourg   (2019-09-13)
Revision as of 11:04, 13 October 2019 by Katya (Talk | contribs)


Guanine inactif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for guanine and guanosine ligands. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923003

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/rna/uncategorized
Parameters
ligandsin-vitro assay : Guanine; in-vivo assay Guanosine
switch_pointThe maximum effect obtained at approximately 500 μM of guanosine