RNA

Part:BBa_K2923008

Designed by: claire husser   Group: iGEM19_Strasbourg   (2019-09-13)
Revision as of 10:40, 13 October 2019 by Katya (Talk | contribs)


Guanine inactif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine (ligand). It is composed of 3 different parts: a twister ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme, this means that the ribozyme part is mutated and the aptazyme lost its capacity to cleave itself. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923003

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/rna/uncategorized
Parameters
ligandsin-vitro assay : Guanine; in-vivo assay Guanosine
switch_pointThe maximum effect obtained at approximately 500 μM of guanosine