Part:BBa_I732957

Ptemplate1 + RBS.ARL2A0301.TERM

Genotype	Character	Name
Top10/pT-TERM	So not express any repressors	CR00
Top10/pT-ARL4A0604	Constitutively express ARL4A0604	CR01
Top10/pT-ARL2A0203	Constitutively express ARL2A0203	CR10
Top10/pTet-ARL4A0604-ARL2A203	Constitutively express ARL4A0604 and ARL2A0203	CR11

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1242
  Illegal EcoRI site found at 4409
  Illegal XbaI site found at 4574
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4409
  Illegal SpeI site found at 1243
  Illegal PstI site found at 1257
  Illegal NotI site found at 1250
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4409
  Illegal XhoI site found at 2276
  Illegal XhoI site found at 3302
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1243
  Illegal EcoRI site found at 4409
  Illegal XbaI site found at 4574
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4409
  Illegal XbaI site found at 4574
  Illegal SpeI site found at 1243
  Illegal PstI site found at 1257
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3448
  Illegal BsaI.rc site found at 4433