Coding

Part:BBa_K1021005

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-08-30)
Revision as of 19:31, 28 October 2013 by E.Holmes (Talk | contribs)

Cre recombinase

The Cre recombinase can be used for site-specific recombination of constructs flanked by lox sites (BBa_J61046). The Cre-Lox recombination system can be used for gene manipulation and as a biosafety mechanism. As a biosafety mechanism, its action is to remove engineered genes flanked by lox sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None